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• Avenida Brasil Episodes

19 October 201251#139PortugalBesides the success in Brazil, Avenida Brasil, is also a big hit with the audience in. In its debut, 24 September 2012, the novel has recorded 13 points and 31.6% audience share, finishing third in the ranking of hearings that day. Week after week, the soap opera continued to record satisfactory levels of audience.

On 15 November 2012 Avenida Brasil recorded 11.4 points and an audience share of 36%, meaning that one in three televisions were watching a telenovela. It was the fourth most watched program each day.

On 3 January 2013 it was registering 15.9 points and an audience share of 36.6%, the largest audience to date. It remained fourth place in the rankings. Timeslot# Ep.PremiereFinalRankSeasonViews averageDatePremiereshareDateFinaleshare.

Avenida Brasil Episodes

6 September 201339%No. 120131.5 millionNational reception The soap opera had a successful debut, becoming increasingly popular, until becoming a great critical and commercial success. It became a favorite subject in the social networks of the internet, being one of the topics most discussed on Twitter, the hashtag reaching the top Trending Topics for each chapter of the story. The night of its final chapter became epic, on Twitter, the hashtag #OiOiOiFinal ranked first in world affairs on Twitter and continued after the end of the exhibition throughout Brazil. In addition to success in social networks, the last chapter brought Brazil to a halt, making the streets of big cities like, Rio de Janeiro and stands deserted. Programs of had segments dedicated to the soap and several other Brazilian television stations also commented on the end of Avenida Brasil.

With many connected TVs, there was a hoax that a blackout could occur in the whole country after the screening of the final episode due to an effect called 'loading ramp' when the novel ends, people resume activities which could generate an overload leaving the country in the dark which did not actually occur. At the close of the soap opera, the telenovela beat its own record. As expected by most critics, the last episode was watched by 80 million people, a very high number, making it the highest rated TV program in 2012 in Brazil.

The group’s original lineup consisted of brothers Brian, Dennis and Carl Wilson, their cousin Mike Love, and their friend Al Jardine. Influenced by jazz-based vocal groups, 1950s rock and roll, and doo-wop, Brian led the band to experiment with several genres ranging from pop ballads to psychedelic and baroque while devising novel approaches to music production and arranging. Flac good vibrations music. Emerging at the vanguard of the “California Sound”, the band’s early music gained international popularity for their distinct vocal harmonies and lyrics evoking a southern California youth culture of surfing, cars, and romance.

Awards and nominations. English title-card.Avenida Brasil has become the most exported telenovela, surpassing (ibid), which was the prior sales leader for other countries.The telenovela has been licensed by more than 150 countries including all of, United States, and, being dubbed into 19 languages. Reception In it received 16.1 points and a 39% audience share for the last chapter, which means more than 1.5 million viewers watched the outcome of the novela. The plot debuted in SIC in September 2012 and was leader of the time in which it was displayed. It was one of the most watched programs in the country, second only to 'Dancin' Days', co-produced by Globo and SIC. The novel also has good viewing figures in, and reached an average of 12 points with peaks of 13.3 in its first chapter, ensuring impressive viewing figures for.

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The number of draft genomes deposited in Genbank from the National Center for Biotechnology Information (NCBI) is higher than the complete ones. Draft genomes are assemblies that contain fragments of misassembled regions (gaps). Such draft genomes present a hindrance to the complete understanding of the biology and evolution of the organism since they lack genomic information.

To overcome this problem, strategies to improve the assembly process are developed continuously. Also, the greatest challenge to the assembly progress is the presence of repetitive DNA regions. This article highlights the use of optical mapping, to detect and correct assembly errors in Corynebacterium pseudotuberculosis. We also demonstrate that choosing a reference genome should be done with caution to avoid assembly errors and loss of genetic information. Next Generation Sequencing (NGS) platforms provide an exponential increase in the amount of data produced in a single assay (high-throughput data).

This approach provided the scientific community with the ability to sequence more genomes at reduced costs. The NGS platforms perform the sequencing through different technologies, which were developed by different companies, such as 454 GS FLX system (Roche); Hiseq paired-end (Illumina); Ion Torrent PGM (Life Technologies); PacBio sequel system(Pacific Biosciences); and MinION (Oxford Nanopore). From these, thousands of genomic projects were created to sequence Bacteria, Archaea, and Eukarya species, viruses, and metagenomes.The main database of these sequences is GenBank maintained by the National Center for Biotechnology Information (NCBI), which in September 2018, contained 153,992 bacterial genomes, most of these being drafts, and only 11,103 sequences (7%) were complete genome sequences. Furthermore, the complete sequences still might have misassemblies due to the presence of repetitive regions, such as ribosomal RNA (rRNA), transposases, phage regions, and plasmids. These errors bias future studies and inferences, such as in comparative genomic or structural genomic analyses, and even ordering of phylogenetically related genomes. Thus, obtaining a more precise and accurate complete genome sequence of an organism is fundamental to understanding its biological and evolutionary characteristics.The assembly problem persists even with the increase in the reads size, sequencing quality, and updates of de novo assembly algorithms.

Another limiting factor to the increase of complete sequences is the lack of trained professionals. However, approaches to support this process have been gaining prominence. For example, the use of SSPACE software to use paired-end reads to create a consensus sequence and perform scaffolding of contigs. Similarly, MapRepeat and riboSeed try to solve the repetitive region’s problem.In order to solve this assembly problem and to improve the generated data, we have the strategy of optical mapping, or Whole Genome Mapping (WGM), which is an approach that uses high-resolution restriction maps to generate the actual orientation of the organism’s genome. It is the main method of large-scale genome analysis that provides complete visualization of the structural genome through a single image. Optical mapping is based on the distance of the restriction sites for high precision map construction.

This is a strategy in which data are obtained with greater precision since it is a physical result of the genome evaluation. This method, combined with the application of de novo assembly methodology, assists in the orientation of contigs.The technique of optical mapping was first developed by Schwartz and collaborators in 1993, with the purpose of studying the chromosomal gene ordering of Saccharomyces cerevisiae. Samad et al., 1995, describe optical mapping as the novel approach for single-molecule DNA analysis using flowering microscopy to identify and estimate its size by the generated images. Since then, several improvements have been added to the technique, especially in the images and algorithms for fragment size estimation. Hence, WGM gained notoriety in several applications, such as in lineage typing in epidemic cases for clinical microbiology,; ordering of contigs generated by de novo assembly; and in the study of inversions, insertions, deletions, duplications, and instability of bacterial genomes. WGM has been successfully performed on very different types of organisms such as bacteria, fungi, plants, and mammals.Regarding the genomic assembly strategy, WGM is an additional method that allows the ordering of the contigs and thus provides a size estimation of the gaps and their positions.

This combination of methods is called a hybrid approach to scaffolding assembly, and it is feasible to acquire a complete genome of high quality and accuracy.The work carried out by Mariano and collaborators in 2016, updated the genome of Corynebacterium pseudotuberculosis 1002 (later deposited as Cp1002B) using the optical mapping technique. Pseudotuberculosis 1002B was the first organism of the Corynebacterium genus that had optical mapping applied in the detection and correction of assembly errors. From the results obtained by Mariano and collaborators in 2016, we decided to investigate another 10 genomes with this strategy, namely: C. Pseudotuberculosis 29156 (Cp29156), C. Pseudotuberculosis I19 (CpI19), C.

Pseudotuberculosis FRC41 (CpFRC41), C. Pseudotuberculosis T1 (CpT1), C. Pseudotuberculosis 31 (Cp31), C. Pseudotuberculosis Cp162 (Cp162), C. Pseudotuberculosis MB302 (CpMB302), C. Pseudotuberculosis CIP52.97 (CpCIP52.97), C.

Pseudotuberculosis MB1 (CpMB11) and C. Pseudotuberculosis 258 (Cp258) (Table ).

Among these genomes, 4 are strains from the biovar ovis and six from the biovar equi. Sequencing and assemblyThe strains deposited by our research group were either re-sequenced (i.e., Cp1002 (1002B), CpI19, Cp31, Cp162, Cp258, CpCIP52.97), or were first sequenced using the Ion Torrent PGM TM platform (i.e., Cp29156, CpMB302, CpMB11, CpT1) (Table ). Different software packages were used for de novo assembly (Table ). Optical mapping analysis: biovar ovis strainsThe strains Cp1002 (CP001809.2) and CpI19 (CP002251.1) (Fig. ) showed an inversion of approximately 1.6 Mb and 1.22 Mb, respectively. It is observed in the regions flanking the first and third clusters of Ribosomal RNA in the CpI19 strain; while in Cp1002, it occurs between the first and fourth clusters.

Figure show the starting and ending points of the inversion, labeled as R1 and R2, respectively. The central block in Fig. Corresponds to the physical restriction map, while the upper and lower blocks represent the in silico restriction map generated by MapSolver TM software. The red regions of the central block (Fig. ) indicate that the same region exists in both the first version (Upper Block) and the updated version (Lower Block). Thus, they show that there was no significant loss between the compared versions in that region.